Investigation Details

Notes on turnaround Times

For blood specimens, the colour of the text within the 'Container' column indicates the required specimen lid colour.
If unsure, click the text within the column for an image (See 'Sample Containers' section for paediatric versions).

Please note that the volumes quoted relate to individual tests.
If multiple tests are ordered, an appropriate increase in sample volume may be required.
Multiple tests for the same specimen type will normally only require a single specimen.

All requests made electronically via the ICE system will automatically include on the request form the type and number of specimens required.

Investigation Search

Test/Profile	Container, Specimen Type & Volume	Additional Information	Department	Turn - around Days	Out of Hrs	Reference
17-hydroxyprogesterone (17-OHP)	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum	For further details click here	Biochemistry Referred Test Barts and the Royal London NHS Trust	11
5HIAA (24hr urine)	Acidified 24hr Urine or Plain 24hr Urine 24hr Urine	For the investigation of carcinoid syndrome. For further details click here.	Biochemistry Referred Test St Helier Hospital	7 to 14
6 thioguanine (6TG)/ Methyl mercaptopurine(Thiopurine metabolites)	EDTA Blood (4 mL)	Separate sample required. Used to monitor azathioprine therapy. For further details click here	Biochemistry Referred Test City Hospital, Birmingham	5
Acid Fast Bacilli (AFB) Culture & Sensitivity	150ml Container Complete EMU	Three complete early morning samples (not MSU's) are required in screw cap containers	Microbiology (7718)	42
ACTH	EDTA Minimum 4mL	For further details click here.	Biochemistry Referred Test Barts and the Royal London NHS Trust	10
Activated Partial Thromboplastin Time Ratio (APTR)	Citrate Blood (2.7 or 1.8 mL)	Sample must be received on day of collection.	Coagulation (7695)	1
Acylcarnitines (plasma or blood spot)	Lithium Heparin or Blood Spot 2 x blood spots Minimum 0.5mL blood	For the investigation of neonatal/infantile hypoglycaemia, organic acidurias, MCAD deficiency. Whole blood can be can be spotted on a Guthrie card from a lithium heparin tube For confirmation of MCADD diagnosis on neonate with positive neonatal screening for MCAD, both blood spots and plasma for acylacarnitines and urine organic acids should be sent to Sheffield	Biochemistry Referred Test Viapath, London (WPH) GOSH, London (RBH and WPH),Queens Square, London (WPH - plasma)Sheffield Children's Hospital (Confirmation screen)	10(Viapath) 14 (GOSH)
ADAMTS-13	Citrate Blood (4 x 2.7 mL)	Must be received on day of collection. Requires four 2.7ml samples. Test performed at UCL.	Coagulation (7695)	56
Albumin	SST Blood (5 mL)		Biochemistry	<1
Alcohol (ethanol)	SST Blood (5 mL)	If a delay of >3hrs is expected before receipt by laboratory, please use Fluoride Oxalate.	Biochemistry	<1
Aldosterone	Lithium Heparin Minimum 1ml plasma	For the investigation of hypertension. More details in the Endocrine Test E-Book. See Renin also.For further details click here	Biochemistry Referred Test Charing Cross Hospital, London	28
Alkaline Phosphatase	SST Blood (5 mL)		Biochemistry	<1
Alkaline Phosphatase Isoenzymes	SST Minimum 250uL serum	GGT may hGGT may help in the differential diagnosis of raised ALP. For further details click here	Biochemistry Referred Test Viapath, London	7 to 14
Allergen Specific IgE (RAST allergy)	SST Blood (5 mL)	More information	Biochemistry Referred Test St Helier Hospital	7 - 28
Alpha 1 antitrypsin (A1AT)	SST (5 mL)	Phenotype will be referred if A1AT is below 1.0 g/L.	Biochemistry	7
Alpha FetoProtein (AFP)	SST (5 mL)	As tumour marker used to monitor germ cell tumours and hepatoma. Note not useful for diagnosis of malignancy.	Biochemistry	<1
ALT	SST Blood (5 mL)		Biochemistry	<1
Aluminium	Trace Metals Blood (5 mL)	For further details click here	Biochemistry Referred Test Royal Surrey County Hospital (BSPS)	5
Amino acids (blood)	Lithium Heparin Minimum sample volume 2-3ml blood (0.5 ml plasma) 0.1 ml plasma (GOSH)	For the investigation of neonatal/infantile hypoglycaemia and other inherited metabolic disorders. Deliver immediately to the laboratory For further details (Viapath) click here For further details (GOSH) click here	Biochemistry Referred Test Viapath, London (WPH) GOSH, London (RBH)	14 (Viapath) 14 - 21 (GOSH)
Amino Acids(urine)	Monovette® Minimum 2ml fresh urine	For the investigation of neonatal/infantile hypoglycaemia and other inherited metabolic disorders. To lab in 2 hours For further details (Viapath) click here For further details of (GOSH) click here	Biochemistry Referred Test Viapath, London (WPH) GOSH, London (RBH)	14 (Viapath) 21- 28 (GOSH)
Ammonia	EDTA Blood (5 mL)	Contact ext:7704 or bleep out of hours staff (bleep 293) before sending. Tube on iced water. Immediate transport to laboratory required. Avoid dirty skin.	Biochemistry Referred Test St Helier Hospital	<1
Amylase	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Androgen Profile (inc. Androstenedione & DHAS)	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood (5 mL)	Includes Testosterone, SHBG, Androstenedione and DHA sulphate. Please select individual tests if the full profile is not necessary. For neonates do NOT use paediatric gel separator tubes as a component of the gel may interfere with analysis. Use plain serum tubes only. The minimum volume for paediatric samples is 0.5 ml of blood. Adult Li Hep and SST specimens with gel separators are acceptable.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 21
Angtiotensin Converting Enzyme (ACE)	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Antenatal Blood Group Serology	6ml EDTA Blood (6 mL)	Sample must be handwritten. Adhesive and addressograph labelled samples will not be accepted.	Blood Bank (7697)	3
Antenatal Virology Screen	SST Blood	More information	Virology (7715)	5
Antibiotic Assay: Tobramycin/ Amikacin/ Bactericidal Levels	SST Blood (5 mL)	More information	Microbiology (7718)	2
Antibody Screen (Virology - Chlamydia, Q fever, Mycoplasma, Legionella, Syphilis, Toxoplasma, ASOT, Helicobacter, Lyme Disease (Borrelia))	SST Blood	More information	Virology (7715)	5
Antidepressants	Lithium Heparin or Plain Blood (5 mL)	Not routinely offered. For ?Toxicity or ?Compliance only. Request to be discussed with Duty Biochemist.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Antieplileptics	Lithium Heparin or Plain Blood (5 mL)	Ethosuximide & Lamotrigine: pre-dose level only. For ?Toxicity or ?Compliance only. For other antiepileptic drugs look under specific name. Request to be discussed with Duty Biochemist.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Antimalarials	Lithium Heparin or Plain Blood (5 mL)	Not routinely offered. For patients on IV quinine only. Request to be discussed with Duty Biochemist.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Antipsychotics	Lithium Heparin or Plain Blood (5 mL)	Not routinely offered. For ?Toxicity or ?Compliance only. Request to be discussed with Duty Biochemist.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Anti-Xa Assay	Citrate Blood (2 x 2.7 mL)	Must be received on day of collection. Requires two 2.7ml samples. Please contact the department if paediatric samples are to be taken. Result only informative if sample taken 3-4 hours post dose.	Coagulation (7695)	3
Apolipoprotein B (ApoB)	SST, Plain or Lithium Heparin Blood (5 mL)	Should be discussed with the Chemical Pathologist prior to request being made on 0118 322 7704 or royalberkshire.biochemistry@nhs.uk	Biochemistry Referred Test St Helier Hospital	7
Ascitic fluid protein, albumin & LDH	Universal Ascitic Fluid (10 mL)	Please request serum levels at the same time as the fluid request. If these tests are required outside normal working hours then please contact the laboratory directly by bleeping 293	Biochemistry Referred Test St Helier Hospital	2
Ascitic/ Peritoneal fluid (C&S)	Polycon Container or Universal Ascitic Fluid (2-3 mL)	Sample storage and transport times upon testing < 2 hours at room temperature.	Microbiology (7718)	24 - 72 hrs
Ascitic/ Peritoneal fluid (Cell count)	Polycon Container or Universal Ascitic Fluid (2-3 mL)	Sample storage and transport times upon testing < 2 hours at room temperature.	Microbiology (7718)	< 2 hours
Ascitic/ Peritoneal fluid (Gram stain)	Polycon Container or Universal Ascitic Fluid (2-3 mL)	Sample storage and transport times upon testing < 2 hours at room temperature.	Microbiology (7718)	< 2 hours
Asialotransferrin	Universal Nasal/Ear Fluid (2 mL)	For the identification of CSF in rhinorrhoea fluid. Also called Tau protein.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Aspartate Transaminase (AST)	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	<1
AT Variants	Citrate Blood (4 x 2.7 mL)	Must be received on day of collection. Requires four 2.7ml samples. Test performed at Addenbrooks.	Coagulation (7695)	56
Bacterial Culture & Sensitivity, Parasites, Rotavirus, Clostridium Difficile	Polycon Container Faeces (1 g)	More information	Microbiology (7718)	5
Beta-2-microglobulin	SST or Plain Blood (5 mL)	For the monitoring of tumour mass and renal function in Myeloma patients.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Beta-hydroxybutyrate	Lithium Heparin Blood (5 mL)	For the investigation of neonatal/infantile hypoglycaemia. The sample should be sent to the laboratory immediately.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Bicarbonate	SST, Plain or Lithium Heparin Blood (5 mL)	Must be received on day of collection. In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Bile acids	SST or Plain Blood (5 mL)	For the diagnosis of Cholestasis of Pregnancy.	Biochemistry Referred Test St Helier Hospital	2
Biotinidase	Lithium Heparin or Blood Spot Blood (5 mL)	For the investigation of inherited metabolic defects with skin problems and epilepsy. For the investigation of adult biotin deficiency suggest vitamin trial. Plasma to be sent on ice to the laboratory within 15 minutes of collection.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 21
Blood and CAPD Culture and Sensitivity	Bactec System Bottles Blood/ CAPD Fluid	More information	Microbiology (7718)	7
Blood Culture (Line Sepsis)		Specimen transport time upon testing must be <4 hours at room temperature. Adults: At least 2 sets from two separate venepuncture. Multiple blood culture sets collected from the same venepuncture are regarded as one, not separate sets. However, in neonates and infants two venepunctures may not always be possible. Please refer to the RBFT Blood Culture Protocol on the intranet.	Microbiology (7718)
Blood Culture (Sepsis)	Blood (See additional)	Specimen transport time upon testing must be <4 hours at room temperature. Adults: At least 2 sets from two separate venepuncture. Multiple blood culture sets collected from the same venepuncture are regarded as one, not separate sets. However, in neonates and infants two venepunctures may not always be possible. Please refer to the RBFT Blood Culture Protocol on the intranet.	Microbiology (7718)
Blood Film	EDTA Blood (4 mL)	A blood film will be laboratory generated if the results and / or clinical details suggest this will be clinically helpful.	Haematology (7693)	2
Blood gases (ABG - includes pH, pO2, pCO2, O2sat, std bicarb, base excess)	Syringe (Heparinised) Arterial Blood	This test is currently unavailable from the lab. Please use other blood gas analysers located around the trust. Contact bleep 293 if you need assistance.	Biochemistry Referred Test St Helier Hospital
BNP (NT-proBNP)	Lithium Heparin Blood (5 mL)	For the investigation of patients with symptoms of heart failure. N.B test only offered for primary care.	Biochemistry Referred Test St Helier Hospital	5
Bone marrow aspirate	Bone marrow slides Blood (5 mL)	Must be agreed with Haematology Consultant/Specialist Registrar	Haematology (7693)	7
Bone marrow trephine	Bone marrow slides Blood (5 mL)	Bone marrow slides Must be agreed with Haematology Consultant/Specialist Registrar	Haematology (7693)	7
Bone Profile	SST, Plain or Lithium Heparin Blood (5 mL)	Includes calcium, phosphate, ALP and albumin. In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
C1-esterase inhibitor	SST or Plain Blood (5 mL)	For the diagnosis of angioneurotic oedema. C4 should be requested at the same time. C1-esterase inhibitor will not be analysed if C4 is normal.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
CA 125 (CA125)	SST or Plain Blood (5 mL)	Follow NICE Ovarian Cancer Guidance CG122 (2011) which states: Measure CA125 in primary care if a woman (especially if 50 or over) reports having any of the following symptoms on a persistent or frequent basis (particularly more than 12 times per month): - persistent abdominal distension (women often refer to this as “bloating”) - feeling full (early satiety) and/or loss of appetite - pelvic or abdominal pain - increased urinary urgency and/or frequency CA125 should also be measured in any woman of 50 or over who has experienced symptoms within the last 12 months that suggest irritable bowel syndrome (IBS), because IBS rarely presents for the first time in women of this age.	Biochemistry Referred Test St Helier Hospital	2
CA 15-3 (CA153)	SST or Plain Blood (5 mL)	For the follow up of breast cancer. Not useful for screening or diagnosis of cancer.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
CA 19-9 (CA199)	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood (5 mL)	For the follow up of known pancreatic and other gastrointestinal cancers. Not useful for screening or diagnosis of cancer.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Caeruloplasmin	SST, Plain or Lithium Heparin Blood (5 mL)	Refer to copper.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Calcitonin	SST or Plain Blood (5 mL)	For the diagnosis of Medullary Carcinoma of the thyroid. Overnight fasting sample required. Inform lab before taking specimen and urgently transport specimen on ice to laboratory once taken.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Calcium	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Calcium	Acidified 24hr Urine 24hr Urine		Biochemistry Referred Test St Helier Hospital	2
Calculus (renal) (stone / calculi)	Universal Calculus	For the investigation of the chemical composition of the calculus	Biochemistry Referred Test Barts and the Royal London NHS Trust	10
Carbamazepine	SST, Plain or Lithium Heparin Blood (5 mL)	Need to know time since last dose. Ideally collect sample shortly before a dose is to be taken.	Biochemistry Referred Test St Helier Hospital	<1
Carbapenemase Producing Enterobacteriaceae (CPE) screen			Microbiology (7718)
CarboxyHaemoglobin/ Methaemoglobin	Lithium Heparin Blood (5 mL)	For hospital users, please note that this test should be carried out on the blood gas analysers available on site at several wards and departments.	Biochemistry Referred Test St Helier Hospital	1
Cardiac Enzymes, Profile	SST, Plain or Lithium Heparin Blood (5 mL)	Includes CK. In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Catecholamines	Acidified 24hr Urine Blood (5 mL)	This test is no longer offered and has been replaced by metadrenalines.	Biochemistry Referred Test St Helier Hospital
CEA	SST or Plain Blood (5 mL)	Not useful for screening or diagnosis of colorectal cancer.	Biochemistry Referred Test St Helier Hospital	2
Cell Markers	EDTA Blood (4 mL)	To be requested on advice of Consultant Haematologist. GP requests should not be sent on Fridays.	Haematology (7693)
Chloride (Cl-)	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	<1
Cholesterol	SST, Plain or Lithium Heparin Blood (5 mL)	No need to be fasting if required in isolation.	Biochemistry Referred Test St Helier Hospital	1
Cholinesterase (serum)	SST or Plain Blood (5 mL)	For the investigation of suxamethonium sensitivity and organophosphate poisoning.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Chromagranin A and B	EDTA Blood (2 x 4 mL)	Requires two 4ml EDTA samples. To be sent on ice to the laboratory within 15 minutes of collection.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Chromium/ Cobalt	Trace Metals Blood (5 mL)	Usually only in secondary care for symptomatic patients post hip prosthesis with ASRTM acetabular cups	Biochemistry Referred Test Barts and the Royal London NHS Trust	21
Clostridium Difficile	Polycon Container or Universal Faeces	Bristol Stool Chart 5 - 7. Transport time should be less than 2hrs. Between 2200 - 0700: Prior approval by the CMM on call is mandatory. Upon collection of the specimen please notify the laboratory of sample's arrival to expedite testing.	Microbiology (7718)
Clotting Screen (INR/APTR)	Citrate Blood (2.7 or 1.8 mL)	Must be received in laboratory on day of collection	Coagulation (7695)	1
CMV PCR	Urine	Please request from the department a 3ml CMV transport medium container.	Virology (7715)
Cold Agglutinins	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood (6 mL)	Sample must be kept at 37 degrees C. Please send patient for blood collection at RBH Pathology Outpatients	Blood Bank (7697)	7 days
Complement (C3 C4)	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Conjugated Bilirubin	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	<1
Copper	SST, Plain or Lithium Heparin Blood (5 mL)	For the investigation of Wilson's disease. Caeruloplasmin should be requested at the same time (except in TPN).	Biochemistry Referred Test Barts and the Royal London NHS Trust	28
Corenal Ulcer	Bed-side inoculated slide and culture Corneal scraping		Microbiology (7718)
Cortisol	Plain 24hr Urine 24hr Urine	Details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	21
Cortisol	SST, Plain or Lithium Heparin Blood (5 mL)	Random levels of limited value. Best investigated by appropriate dynamic test. More details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	2
C-Peptide	Lithium Heparin Blood (5 mL)	Request together with Insulin for the investigation of hypoglycaemia in adults and children. More details in the Endocrinology E-Book.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
C-Reactive Protein (CRP)	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	<1
Creatinine	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Creatinine Clearance	Plain 24hr Urine 24hr Urine	A paired serum sample for creatinine is necessary for creatinine clearance calculation.	Biochemistry Referred Test St Helier Hospital	2
Creatinine Kinase (CK)	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Crossmatch	6ml EDTA Blood (6 mL)		Blood Bank (7697)
Cryoglobulins	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood (5 mL)	Patient must attend South Block Blood Tests clinic if they require this test.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
CSF Culture and Sensitivity	Universal CSF (0.5 mL)	More information	Microbiology (7718)
CSF Glucose	Fluoride Oxalate CSF (0.5 mL)	A blood (fluoride oxalate - grey top) sample for glucose should be taken at the same time. Specimen must NOT be sent via the pneumatic air-tube system.	Biochemistry Referred Test St Helier Hospital	<1
CSF Lactate	Fluoride Oxalate CSF (0.5 mL)	For the investigation of inherited metabolic disorders with lactic acidaemia. Paired blood lactate necessary.. Fluoride tubes should be brought to lab on ice ASAP. Specimen must NOT be sent via the pneumatic air-tube system.	Biochemistry Referred Test Barts and the Royal London NHS Trust
CSF Oligoclonal bands	Universal CSF (0.5 mL)	A blood sample (SST or Plain tube) should be taken at the same time. Specimen must NOT be sent via the pneumatic air-tube system.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21
CSF Protein	Universal CSF (0.5 mL)	Blood-stained CSF is not suitable for this test. Specimen must NOT be sent via the pneumatic air-tube system.	Biochemistry Referred Test St Helier Hospital	<1
CSF Xanthochromia	Universal CSF (0.5 mL)	Sample should be collected at least 12h post onset of symptoms. Collect at least 1.0 ml of the fourth sample. Protect sample from light. To arrive in Biochemistry within one hour of collection. Please highlight request on form and bleep 293 before sending specimen. This test is only offered during normal working hours. If you require this test at any other time, please contact the on call consultant biochemist via the switchboard. Sample must NOT be sent via the pneumatic air-tube system.	Biochemistry Referred Test St Helier Hospital	2
Culture for Atypical Mycobacteria including MAI	Citrate Blood	4.5ml glass vacutainers only Two 5mL blood samples are required. Referred to Specialist reference laboratory.	Microbiology (7718)	42
Cyclosporin/ Ciclosporin	EDTA Blood (4 mL)	Separate sample required. Sample should be taken pre-dose or at 12 hours post dose. Results are available sooner via Oxford's 'Proton' system.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7
Cystic fibrosis carrier status	EDTA Blood (4 mL)	Only useful if sweat test is positive or persistently equivocal. If there is a family member with a CF or known carrier status, please provide name, relationship and mutation.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Cystine	Acidified 24hr Urine 24hr Urine		Biochemistry Referred Test Barts and the Royal London NHS Trust	21
Cystine Screening Test	Acidified 24hr Urine Blood (5 mL)	This test is no longer offered. Refer to Cysteine	Biochemistry Referred Test Barts and the Royal London NHS Trust	7
Cytogenetics	Lithium Heparin Blood (5 mL)	This is a reference laboratory test	Haematology (7693)	21
Cytology	60ml Dry Vial Random Urine (10 mL)	Must be received in laboratory on day of collection	Cytology	3
Cytology	60ml Dry Vial Sputum	Must be received in laboratory on day of collection	Cytology	3
D-Dimers (FDPs)	Citrate Blood (2.7 or 1.8 mL)	Must be received in laboratory on day of collection	Coagulation (7695)	1
Digoxin	SST, Plain or Lithium Heparin Blood (5 mL)	Sample should be taken 6-8 hours post dose. A potassium level is useful for the interpretation of digoxin.	Biochemistry Referred Test St Helier Hospital	<1
Direct Coombs Test	6ml EDTA Blood (6 mL)		Blood Bank (7697)	2
Discitis	Polycon Container or Universal Disc Biopsy	Transport time less than 2hrs at room temperature and 2-48hrs refigerated.	Microbiology (7718)
Elastase	Universal Faeces (1 g)	For the investigation of malabsorption.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Endophtlamitis or surgery specimens (C&S)	Syringe Aqueous humour tap, vitreal biopsy	Syringe without needle (use steile syringe cap). Transport time must be < 2 hours at room temperature.	Microbiology (7718)	24 - 72 hrs
Erythropoietin (EPO)	SST or Plain Blood (5 mL)		Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 24
ESR	EDTA Blood (4 mL)	Both FBC & ESR can be done on one fully-filled 4ml lavender bottle.	Haematology (7693)	2
EV PCR for CSF	Polycon Container or Universal		Microbiology (7718)	3
Eye Swab, Culture and Sensitivity, Virus and Chlamydia	Swab	More information	Microbiology (7718)
Factor Assay	Citrate Blood (2 x 2.7 mL)	Requires two 2.7ml samples. Please contact the department if paediatric samples are to be taken.	Coagulation (7695)	21
Factor V Leiden (FVL)	EDTA Blood (4 mL)	Test performed at North Middlesex Hospital	Coagulation (7695)	56
Faecal Calprotectin	Polycon Container Faeces (1 g)	May be useful to help differentiate non-inflammatory bowel disorders (e.g. irritable bowel syndrome) and inflammatory bowel disease (e.g. ulcerative colitis and Crohn's disease). Calprotectin will also be elevated in some cases of GI tract malignancy (e.g. colorectal cancer). Please only request after discussion with Gastroenterology or by following the protocol.	Biochemistry Referred Test St Helier Hospital	7
Faecal Occult Blood (FOB)	Polycon Container Faeces (1 g)	From November 2014 this test no longer routinely offered, except for Paediatric requests.	Biochemistry Referred Test St Helier Hospital	2 to 4
Ferritin	SST or Plain Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Fibrinogen	Citrate Blood (2.7 or 1.8 mL)	Must be received in laboratory on day of collection	Coagulation (7695)	1
Flecainide	Plain or EDTA Blood (4 mL)	Only useful in children. Sample to be taken pre-dose or 5hrs post-dose. Please discuss with Duty Biochemist.	Biochemistry Referred Test Barts and the Royal London NHS Trust	2 to 4
Flu A/B PCR	Swabs in VTM Nose and Throat Swabs. Nasal aspirate/ washes	Specimen transport time < 2 hrs at room temperature and 2 - 72 hrs refrigerated. Between 2200 - 0700: Prior approval by the CMM on call is mandatory. Upon collection of the specimen please notify the laboratory of sample's arrival to expedite testing.	Virology (7715)	2.5hrs
Folate	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Follicle Stimulating Hormone (FSH)	SST, Plain or Lithium Heparin Blood (5 mL)	Stage of cycle is useful for interpretation. Not useful when on OCP or HRT.	Biochemistry Referred Test St Helier Hospital	2
Free fatty acids	Lithium Heparin Blood (5 mL)	For the investigation of neonatal/infantile hypoglycaemia. The sample should be sent to the laboratory immediately (less than 30 mins).	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Fructosamine	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	7
FT3	SST, Plain or Lithium Heparin Blood (5 mL)	Not routinely measured. More details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	2
FT4	SST, Plain or Lithium Heparin Blood (5 mL)	Details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	2
Full Blood Count (FBC)	EDTA Blood (4 mL)	Both FBC & ESR can be done on one fully-filled 4ml lavender bottle.	Haematology (7693)	2
G6PD	EDTA Blood (4 mL)	For the diagnosis of G6PD deficiency. Interferences: Leukocytes & platelets: cause interference if present beyond normal levels. Copper & Sulphate ions: have been shown to decrease observed levels of G6PD. Higher levels of G6PD are found after a haemolytic crisis as reticulocytes have higher G6PD levels than mature cells.	Biochemistry Referred Test St Helier Hospital	2 to 4
Galactose-1-phosphate uridyl transferase	Lithium Heparin Blood (5 mL)	For the diagnosis of Galactosaemia. Please discuss with Duty Biochemist.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Gamma GT	SST, Plain or Lithium Heparin Blood (5 mL)	Not usually helpful if other LFTs significantly abnormal. Helpful in finding out the source of raised Alkaline phosphatase.	Biochemistry Referred Test St Helier Hospital	<1
Gastric Washings for AFB Culture	Gastric Washings	Please contact the department in advance for a specimen container and to make arrangments.	Microbiology (7718)	5
Gastrin	EDTA Blood (4 mL)	Requires four 4ml EDTA samples. To be sent on ice to the laboratory within 15 minutes of collection. Overnight fasting necessary. Must stop proton pump inhibitors for 2 weeks and H2 antagonists for three days. Do not request all gut hormones if only gastrin is necessary.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Genital HSV	Virus Transport Media	Transport Time less than 2 hours.	Virology (7715)	3
Genital Specimens, Culture and Sensitivity	Transwab Swab	More information	Microbiology (7718)
Genital Swabs, Chlamydia Detection	Chlamydia Swab Swab	Specimens should be collected using the transport kits provided. A swab from the endocervix is the specimen of choice for females. Include the details of the anatomical site, relevant clinical information, age and EDD if pregnant.	Microbiology (7718)
Gentamycin Assay-TDM	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood	Please refer to IV gentamicin/IV teicoplanin/IV vancomycin guide line on the RBFT Intranet.	Microbiology (7718)
Glucagon	EDTA Blood (4 mL)	Requires four 4ml EDTA samples. To be sent on ice to the laboratory within 15 minutes of collection. Overnight fasting necessary. Must stop proton pump inhibitors for 2 weeks and H2 antagonists for three days. Do not request all gut hormones if only gastrin is necessary.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Glucose (as part of profile)	SST, Plain or Lithium Heparin Blood (2 mL)	Time limit for acceptable result is two hours from collection.	Biochemistry Referred Test St Helier Hospital	<1
Glucose (as single test)	Fluoride Oxalate Blood (2 mL)		Biochemistry Referred Test St Helier Hospital	<1
Glucose Tolerance Test (GTT)	Fluoride Oxalate Blood (2 mL)	Click for GP information or for patient information.	Biochemistry Referred Test St Helier Hospital	1
Group & Save	6ml EDTA Blood (6 mL)	Sample must be handwritten. Adhesive and addressograph labelled samples will not be accepted.	Blood Bank (7697)	2
Growth hormone	SST or Plain Blood (5 mL)	Random measurement not useful for investigation of low stature. Best investigated by appropriate dynamic test or IGF-1. More details in the Endocrinology E-Book.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Gut hormones	EDTA Blood (4 x 4 mL)	Requires four 4ml EDTA samples. To be sent on ice to the laboratory within 15 minutes of collection. Overnight fasting necessary. Must stop proton pump inhibitors for 2 weeks and H2 antagonists for three days. Do not request all gut hormones if only gastrin is necessary.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Haematinics (Ferritin, B12, Folate)	SST or Plain Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Haemochromatosis gene	EDTA and SST Blood (See additonal info)	Request only in cases with haematinics suggestive of haemochromatosis. Two x 4mL EDTA tubes and one 2mL SST tube necessary.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Haemoglobin A1c (HbA1c)	EDTA Blood (4 mL)		Biochemistry Referred Test St Helier Hospital	3
Haemoglobinopathy Screen	EDTA Blood (4 mL)		Haematology (7693)	3
Haptoglobin	SST Blood (5 mL)	For the investigation of haemolysis	Biochemistry Referred Test St Helier Hospital	7
H-bodies (HbH Disease)	EDTA Blood (4 mL)	Non UKAS accredited test.	Haematology (7693)	2
HDL Cholesterol	SST, Plain or Lithium Heparin Blood (5 mL)	Overnight fasting sample required (12 - 14 hours). Water can be taken.	Biochemistry Referred Test St Helier Hospital	1
Heliobacter Antibody	SST Blood		Virology (7715)	3
Hepatitis Serology (A, B, C)	SST Blood	A 6ml SST sample is required. Hepatitis A IgM is measured only in patients less than 70 yrs old.	Virology (7715)	2
HIV Antibodies	SST Blood	More information	Virology (7715)	2
HIV-1/2 antibodies and HIV-1 p24 antigens	SST Blood	Transport time < 2 hrs. Between 2200 - 0700: Prior approval by the CMM on call is mandatory. Upon collection of the specimen please notify the laboratory of sample's arrival to expedite testing.	Virology (7715)
HLA B27	6ml EDTA Blood (6 mL)		Blood Bank (7697)	21
Homocysteine	EDTA Blood (4 mL)	As a risk factor for cardiovascular disease. Immediate transport to laboratory required	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 42
Homocysteine	Universal Random Urine (20 mL)	For the investigation of patients with possible homocystinuria.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Homovalinic acid	Universal Random Urine (20 mL)	For the investigation of children with possible neuroblastoma. Send to the laboratory immediately for acidification. More details in the Endocrinology E-Book.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
HSV PCR for CSF	Polycon Container or Universal	This is a reference lab test.	Microbiology (7718)	3
Human Chorionic Gonadotropin (hCG)	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	<1
IgE	SST Blood (5 mL)	For the investigation of allergy.	Biochemistry Referred Test St Helier Hospital	7
IGF-1	SST or Plain Blood (5 mL)	For the investigation of growth hormone disorders. More details in the Endocrinology E-Book.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Immunoglobulins (IgG, IgA, IgM)	SST Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Immunophenotyping	EDTA Blood (4 mL)	This is a reference laboratory test	Haematology (7693)	7
Immunoreactive Trypsin	Guthrie Spot Blood	For the investigation of cystic fibrosis. In children older than 3 months do sweat test instead.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Infectious Mononucleosis (IM) Screen	EDTA Blood (4 mL)		Haematology (7693)	2
INR	Citrate Blood (2.7 or 1.8 mL)	Must be received in laboratory on day of collection	Coagulation (7695)	1
Insulin	Lithium Heparin Blood (5 mL)	Together with C-peptide for the investigation of hypoglycaemia in adults and children (plasma glucose has to be <2.5 mmol/L). For the investigation of insulin resistance in children (insulin only, whatever the plasma glucose level). More details in the Endocrinology E-Book.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Iron	SST, Plain or Lithium Heparin Blood (5 mL)	A request for Iron will usually include Iron, Transferrin and Transferrin Saturation	Biochemistry Referred Test St Helier Hospital	<1
Joint Fluid/ Aspirate (C&S)	Polycon Container or Universal Joint Fluid/ Aspirate	Sample storage and transport times upon testing < 24 hours at room temperature.	Microbiology (7718)	24 - 72 hrs (neg) <5 days (pos)
Joint Fluid/ Aspirate (Gram Stain)	Polycon Container or Universal Joint Fluid/ Aspirate	Sample storage and transport times upon testing < 24 hours at room temperature.	Microbiology (7718)	< 2 hours
Kleihauer	6ml EDTA Blood (6 mL)		Blood Bank (7697)	2
Lactate	Fluoride Oxalate Blood (2 mL)	Contact ext:7704 or bleep out of hours staff (bleep 293) before sending. Immediate transport to laboratory required.	Biochemistry Referred Test St Helier Hospital	<1
Lactate dehydrogenase (LDH)	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Lead (blood)	EDTA Blood (4 mL)	Separate sample required. For the investigation of lead exposure.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Lipid profile	SST, Plain or Lithium Heparin Blood (5 mL)	Fasting (overnight 12-14hrs). triglyceride, cholesterol [if the cholesterol is greater than 6.5 mmol/L, then HDL-cholesterol will also be measured]	Biochemistry Referred Test St Helier Hospital	1
Liquid Pus and Other Fluid Specimen, Culture and Sensitivity	Fluid	More information	Microbiology (7718)
Lithium	SST or Plain Blood (5 mL)	Taken 12 hours post dose. This can not be a Lithium Heparin sample.	Biochemistry Referred Test St Helier Hospital	<1
Liver Function Tests (LFT), (profile)	SST, Plain or Lithium Heparin Blood (5 mL)	Includes total protein, albumin, globulins, alkaline phosphatase, ALT, total bilirubin. In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Lupus Anticoagulant	Citrate Blood (4 x 2.7 mL)	Must be received on day of collection. Requires four 2.7ml samples. Please contact the department if paediatric samples are to be taken.	Coagulation (7695)	56
Luteinizing Hormone (LH)	SST, Plain or Lithium Heparin Blood (5 mL)	State day in cycle or menstrual status. Not useful in determining menopausal status.	Biochemistry Referred Test St Helier Hospital	2
Magnesium	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Malaria Parasites	EDTA Blood (4 mL)		Haematology (7693)	1
Manganese	Trace Metals Blood (5 mL)	For assessment of manganese status in TPN patients please consult the TPN team	Biochemistry Referred Test Barts and the Royal London NHS Trust	21
Metadrenalines	Acidified 24hr Urine or Plain 24hr Urine 24hr Urine	Only two 24hr urines are necessary for the diagnosis of phaechromocytoma or neuroblastoma, preferrably when the patient is symptomatic. Random urines are accepted in very small children. More details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	7 to 14
Metadrenalines	Lithium Heparin Blood (5 mL)	Not routinely offered unless patient cannot produce urine. More details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	14 to 21
Microalbumin/ Urine albumin:creatinine ratio	Monovette® Random Urine (10 mL)		Biochemistry Referred Test St Helier Hospital	3
Microbiology, Urine Culture and Sensitivity	Boric Monovette® Mid-Stream Urine	Monovette®. Please specify MSU, CSU etc. and include clinical details, the date and time of collection and previous and intended antibiotic therapy. The sample should be refrigerated before transport to the department. If examination for Leptospira or Schistosomes is required, please contact the department first.	Microbiology (7718)	3
Molecular Genetics	EDTA Blood (4 mL)	This is a reference laboratory test	Haematology (7693)	42
MRSA Screen	Transwab Nose/Throat	For all admitted patients	Microbiology (7718)	1 or 2
MTHFR Gene Mutation	EDTA Blood (4 mL)	Test performed at North Middlesex Hospital	Coagulation (7695)	56
Mucopolys. (urine GAG)	Universal Random Urine (20 mL)	For the investigation of mucopolisaccharidoses.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Mycobacterium tuberculosis (MTB) (Culture)			Microbiology (7718)
Mycobacterium tuberculosis (MTB) (Microscopy)			Microbiology (7718)
Mycobacterium tuberculosis (MTB) (PCR)	Polycon Container or Universal Sputum BAL/NBL, if performed	Transport time must be <2hrs at room temperature. Between 2200 - 0700: Prior approval by the CMM on call is mandatory. Upon collection of the specimen please notify the laboratory of sample's arrival to expedite testing.	Microbiology (7718)
Mycology - Fungal Culture - Skin Nail Hair	60ml Dry Vial Tissue	More information	Microbiology (7718)	21
Necrotising skin and soft tissue infection specimens e.g, Necrotising fascilltis or incision and drainage of abscesses	Polycon Container or Universal Tissue Specimen or Pus	Tranport time upon testing must be < 2 hours at room temperature and 2 - 72 hours refigerated.	Microbiology (7718)
Neurone-specific enolase	SST or Plain Blood (5 mL)	For the follow-up of APUD tumours and small-cell bronchial carcinoma	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Oestradiol	SST, Plain or Lithium Heparin Blood (5 mL)	Should not be measured to monitor HRT except in subjects with implant. Levels may be misleading during menopausal transition. Rely on FSH.	Biochemistry Referred Test St Helier Hospital	2
Oligosaccharides	Universal Random Urine (20 mL)	For the investigation of oligosaccharidoses.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Organic Acids	Universal Random Urine (20 mL)	Useful in the investigation of certain inherited errors of metabolism.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Orthopaedic Impant-associated infections	Polycon Container or Universal Tissue Biopsy 1 x 0.5 x 0.5 cm3	Transport time less than 2hrs at room temperature and 2-48hrs refigerated. 2 biopsies from the inside of the capsule prior to any manipulation of the prosthesis, (One specimen/ polycon or universal container) 3 biopsies from the Interface membrane, the membrane between the prosthesis and the bone (One specimen/ polycon or universal container) Debridement specimens from fracture fixation operations or prosthetic joint washout procedures (One specimen/ polycon or universal container)	Microbiology (7718)
Orthopaedic Implant-associated infections	Polycon Container or Universal Synovial Fluid	Transport time less than 2hrs at room temperature and 2-48hrs refigerated. Preoperative aseptically collected SF, in theatre or under X-ray guidance in sterile polycon/universal container. Intra-operative joint aspirate before incision of the joint capsule in sterile polycon/universal container.	Microbiology (7718)
Osmolality	SST Blood (5 mL)	Details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	1
Osmolality	Monovette® Random Urine (10 mL)	Details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	2
Oxalate	Acidified 24hr Urine 24hr Urine	For the investigation of patients with renal stones.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21
P1NP	Lithium Heparin or Plain Blood (5 mL)	For the follow up of patients with osteoporosis. This test substitutes P1CP, which is no longer offered.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Pancreatic Polypeptide	Lithium Heparin + trasylol Blood (5 mL)	Refer to gut hormones. Special sample tube required.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Paracetamol	SST, Plain or Lithium Heparin Blood (5 mL)	Please consider timing in relation to OD. Only one measurement is necessary (unless first measurement was before 4 hours post dose).	Biochemistry Referred Test St Helier Hospital	<1
Parathyroid Hormone (PTH)	EDTA Blood (4 mL)	Separate EDTA sample required	Biochemistry Referred Test St Helier Hospital	2
Perinasal Swab, Culture for Bordetella Pertussis (Whooping Cough)	Pernasal Swab Swab	More information	Microbiology (7718)
Peritoneal Dialysate (PD) Fluid Cell Count	Polycon Container or Universal PD Fluid (2-3 mL)	Specimen transport and storage <2 hours at room temperature. PMN cells disingtegrate (32% in 1 hour and 50% in 2 hours)	Microbiology (7718)	<2hrs
Phenobarbital	SST or Plain Blood (5 mL)	Preferred time is pre-dose.	Biochemistry Referred Test St Helier Hospital	<1
Phenylalanine	Guthrie Spot Blood	For the follow up of known PKU patients	Biochemistry Referred Test St Helier Hospital	5 to 10
Phenytoin	SST, Plain or Lithium Heparin Blood (5 mL)	Preferred time is pre-dose.	Biochemistry Referred Test St Helier Hospital	<1
Phosphate	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Phosphate	Acidified 24hr Urine 24hr Urine		Biochemistry Referred Test St Helier Hospital	2
Platelet Aggregation Studies	Citrate Blood (4 x 2.7 mL)	Must be received on day of collection. Requires four 2.7ml samples. Please liase with the department if samples are to be taken. Test performed at Haemophilia Centre, Churchill Hospital.	Coagulation (7695)	56
Platelet Antibodies	Blood	Contact laboratory for details	Blood Bank (7697)	21
Platelet Function Analysis (PFA-100)	Citrate Blood (4 x 2.7 mL)	Must be received on day of collection. Requires four 2.7ml samples. Please liase with the department if samples are to be taken. Test performed at Haemophilia Centre, Churchill Hospital.	Coagulation (7695)	56
Platelet Function Test	Citrate Blood (4 x 2.7 mL)	Must be received on day of collection. Requires four 2.7ml samples. Please liase with the department if samples are to be taken. Test performed at Haemophilia Centre, Churchill Hospital.	Coagulation (7695)	56
Pleural Culture and Sensitivity	Fluid	More information	Microbiology (7718)
Pleural Fluid Glucose	Fluoride Oxalate Pleural Fluid (2 mL)	A fresh sample is required	Biochemistry Referred Test St Helier Hospital	1
Pleural fluid protein, pH and LDH	Universal Pleural Fluid (10 mL)	A fresh sample should be sent for pH. If these tests are required outside normal working hours then please contact the laboratory directly by bleeping 293.	Biochemistry Referred Test St Helier Hospital	1
Porphyrins	Universal Random Urine (20 mL)	Sample should be protected from light e.g. with aluminium foil.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Porphyrins	Polycon Container Faeces (1 g)	Sample should be protected from light e.g. with aluminium foil.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21
Porphyrins (red cell)	EDTA Blood (4 mL)	Separate sample required. Sample should be protected from light e.g. with aluminium foil.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Post-Hep B Vaccine Immune Status	SST Blood	More information	Virology (7715)
Potassium (K+)	SST, Plain or Lithium Heparin Blood (5 mL)	Must be received in laboratory on day of collection. In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Pregnancy Test	60ml Dry Vial Urine	More information	Microbiology (7718)	2
Progesterone	SST, Plain or Lithium Heparin Blood (5 mL)	Useful only if collected during mid-luteal phase - day 21 - in 28 days cycles or estimated 7 days before period in long or short cycles. Random levels are not useful. Not helpful in amenorrhoea.	Biochemistry Referred Test St Helier Hospital	2
Prolactin	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Prostate Specific Antigen (PSA)	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Protein	Monovette® Random Urine (10 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Protein Electrophoresis	SST or Plain Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	7
Protein S Free Antigen (PSFA)	Citrate Blood (4 x 2.7 mL)	Must be received on day of collection. Requires four 2.7ml samples. Please contact the department if paediatric samples are to be taken.	Coagulation (7695)	56
Prothrombin Gene Mutation (PGM)	EDTA Blood (4 mL)	Test performed at North Middlesex Hospital	Coagulation (7695)	56
Pyridoxine	Lithium Heparin Blood (5 mL)	Refer to Vitamin B6	Biochemistry Referred Test Barts and the Royal London NHS Trust
Red Cell Folate (RCF)	Blood (5 mL)	Red cell folate testing has been withdrawn as from 18/3/13 as it is now considered to have no benefit over serum folate as an indication of folate deficiency. Folate replacement therapy should be started on the basis of low serum folate alone.	Biochemistry Referred Test St Helier Hospital
Reducing Substances (FRS)	Polycon Container Faeces (1 g)	Must be received in laboratory within 3 hours of collection. Mark requests as 'URGENT '	Biochemistry Referred Test St Helier Hospital	10
Reducing Substances (URS)	Monovette® Random Urine (10 mL)	Must be received in laboratory within 3 hours of collection. Mark requests as 'URGENT '	Biochemistry Referred Test St Helier Hospital	10
Renal Profile	SST, Plain or Lithium Heparin Blood (5 mL)	Includes sodium, potassium, bicarbonate, urea, creatinine, calcium, albumin, urate & phosphate.	Biochemistry Referred Test St Helier Hospital	<1
Renin	Lithium Heparin Blood (5 mL)	Lying and ambulatory samples necessary for investigation of primary hyperaldosteronism. Random sample only for monitoring fluorocortisone replacement in CAH. Sample should be sent to Biochemistry within half an hour of collection. More details in the Endocrinology E-Book.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Respiratory syncytial virus (RSV) Antigen	NPA collection Kit Nasal Pharyngeal Aspirate	More information Between 2200 - 0700: Prior approval by the CMM on call is mandatory. Upon collection of the specimen please notify the laboratory of sample's arrival to expedite testing.	Virology (7715)	<2 hrs
Reticulocytes	EDTA Blood (4 mL)	Must be received in laboratory on day of collection	Haematology (7693)	2
Rheumatoid Factor	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Rubella Antibodies	SST Blood	More information	Virology (7715)
Salicylate	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	<1
Selenium	Trace Metals Blood (5 mL)	For the assessment of selenium status in patients with special diets (eg. PKU). In long term TPN patients (>3 weeks, once every 4 weeks).	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Serum free light chains (SFLC)	SST Blood (5 mL)	Not routinely offered unless for monitoring of known Multiple Myeloma patients by Haematology or Renal consultants.	Biochemistry Referred Test St Helier Hospital	14
Sex Hormone Binding Globulin (SHBG)	SST Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	7
Sickle Test (Solubility)	EDTA Blood (4 mL)	Both FBC & the Sickle Test can be done on one fully-filled 4ml lavender bottle.	Haematology (7693)	1
Sickle/Thalassaemia screen	EDTA Blood (4 mL)		Haematology (7693)	1
Small Biopsy	Cellstor Tissue		Histology	7
Sodium (Na+)	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Somatostatin	EDTA Blood (4 mL)	Requires four 4ml EDTA samples. To be sent on ice to the laboratory within 15 minutes of collection. Overnight fasting necessary. Must stop proton pump inhibitors for 2 weeks and H2 antagonists for three days. Do not request all gut hormones if only gastrin is necessary.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Sputum Culture and Sensitivity	60ml Dry Vial Sputum	More information	Microbiology (7718)
Sputum for Acid Fast Bacilli Culture	60ml Dry Vial Sputum	More information	Microbiology (7718)
Steroid profile	Plain 24hr Urine 24hr Urine	For the investigation of newborns with ambiguous genitalia.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
STS (Syphilis Screen)	SST Blood	More information	Virology (7715)	7
Swab Samples (wound, ulcer, skin lesion etc.) Culture and Sensitivity/Virus Culture	Swab	More information	Microbiology (7718)
Sweat Chloride	Wescor Macroduct Collector Sweat	For the diagnosis of Cystic Fibrosis.	Biochemistry Referred Test St Helier Hospital	2
Tacrolimus	EDTA Blood (4 mL)	Separate sample required. Pre dose level necessary.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7
Tau-protein	Universal Nasal/Ear Fluid (2 mL)	Refer to asialotransferrin	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Teicoplanin Assay-TCM	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood	Please refer to IV gentamicin/IV teicoplanin/IV vancomycin guide line on the RBFT Intranet.	Microbiology (7718)
Testosterone	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Theophylline	SST, Plain or Lithium Heparin Blood (5 mL)	Ideally collect samples 4 - 6 hours post dose.	Biochemistry Referred Test St Helier Hospital	<1
Thiopurine methyl transferase (TPMT)	EDTA Blood (4 mL)	Separate sample required. Analysed prior to azathioprine therapy.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Throat Swab, Culture and Sensitivity	Transwab Swab	More information	Microbiology (7718)
Throat Swab, Virus Culture	Virus Transport Swab	More information	Virology (7715)
Thrombophilia Screen	Citrate Blood (4 x 2.7 mL)	Includes AT III, Protein C, Protein S Free Antigen and Lupus Anticoagulant - must be received on day of collection. Requires four 2.7ml or six 1.7ml tubes. Please contact the department if paediatric samples are to be taken. Taken in conjunction with an anti-cardiolipin antibody test.	Coagulation (7695)	28
Thyroglobulin	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood (5 mL)	For the follow up of papillary and follicular thyroid carcinoma. TSH should be supressed. Thyroglobulin anti-bodies may interfere.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Thyroid-stimulating Hormone (TSH)	SST, Plain or Lithium Heparin Blood (5 mL)	TSH will be measured as first line test. FT4 or FT3 will be measured as appropriate. Relevant clinical details are vital. More details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	2
Total Bilirubin	SST Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Total Protein	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	<1
Toxicology	Universal Random Urine (20 mL)	Contact Department for Turnaround Time. All requests that have, or are likely to have, medico/legal consequences must be accompanied by a Chain of Custody form.	Biochemistry Referred Test St Helier Hospital	5
Transferrin	SST, Plain or Lithium Heparin Blood (5 mL)		Biochemistry Referred Test St Helier Hospital	2
Transferrin Glycoforms	SST, Plain or Lithium Heparin Blood (5 mL)	For the investigation of inherited metabolic disorders with dysmorphism (not alcoholism).	Biochemistry Referred Test Barts and the Royal London NHS Trust	28 to 42
Transketolase (RBC)	Please contact the laboratory (7704) to discuss Blood	No longer routinely offered	Biochemistry Referred Test Barts and the Royal London NHS Trust
Triglyceride (Fasting)	SST, Plain or Lithium Heparin Blood (5 mL)	Overnight fasting sample required (12 -14 hours). Water can be taken. No alcohol for 12 hours.	Biochemistry Referred Test St Helier Hospital	<1
Troponin T (High sensitivity TNT)	Lithium Heparin Blood (5 mL)	Sample should be taken >2 hours post onset of symptoms. Result interpretation information available from Cardiology. A separate specimen will improve turnaround.	Biochemistry Referred Test St Helier Hospital	<1
Trypanosomes	EDTA Blood (4 mL)		Haematology (7693)	2
Tryptase	Plain or EDTA Blood (4 mL)	Three specimens necessary: during episode and 4 and 24 hours after.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Urea	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Urea & Electrolytes	Plain 24hr Urine 24hr Urine		Biochemistry Referred Test St Helier Hospital	2
Urea & Electrolytes	Monovette® Random Urine (10 mL)		Biochemistry Referred Test St Helier Hospital	2
Urea & Electrolytes (U&E), Profile	SST, Plain or Lithium Heparin Blood (5 mL)	Includes sodium, potassium, creatinine, urea. In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Uric Acid	Plain 24hr Urine 24hr Urine	No Preservative	Biochemistry Referred Test St Helier Hospital	2
Uric Acid	SST, Plain or Lithium Heparin Blood (5 mL)	In patient turn around time <4hrs. Out patient turn around time within 24 hours.	Biochemistry Referred Test St Helier Hospital	<1
Urine Albumin to Creatinine ratio (ACR) / Microalbumin	Monovette® Random Urine (10 mL)		Biochemistry Referred Test St Helier Hospital	2
Urine antigen tests for Legionella and Pneumococcus	Polycon Container or Universal Urine	Transport and stored at room temperature. Between 2200 - 0700: Prior approval by the CMM on call is mandatory. Upon collection of the specimen please notify the laboratory of sample's arrival to expedite testing.	Virology (7715)	<2 hrs
Urine Protein	Plain 24hr Urine 24hr Urine		Biochemistry Referred Test St Helier Hospital	2
Urine Protein electrophoresis a.k.a Bence Jones Protein (BJP)	Monovette® Early Morning Urine (10 mL)		Biochemistry Referred Test St Helier Hospital	7
Urine Protein:Creatinine Ratio (PCR)	Monovette® Random Urine (10 mL)		Biochemistry Referred Test St Helier Hospital	2
Valproate	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood (5 mL)	Routine monitoring not useful as concentration and clinical effect are not correlated. Please discuss with Duty Biochemist.	Biochemistry Referred Test Barts and the Royal London NHS Trust	7 to 14
Vancomycin Assay-TDM	Adult - SST (Serum) Paediatrics - Plain tube (no gel) Minimum 0.25mL serum Blood	Please refer to IV gentamicin/IV teicoplanin/IV vancomycin guide line on the RBFT Intranet.	Microbiology (7718)
Vancomycin Resistant Enterococcus (VRE) screen			Microbiology (7718)
Varicella Zoster	SST Blood		Virology (7715)	2
Vasoactive Intestinal Peptide (VIP)	EDTA Blood (4 mL)	Requires four 4ml EDTA samples. To be sent on ice to the laboratory within 15 minutes of collection. Overnight fasting necessary. Must stop proton pump inhibitors for 2 weeks and H2 antagonists for three days. Do not request all gut hormones if only gastrin is necessary.	Biochemistry Referred Test Barts and the Royal London NHS Trust
Very long chain fatty acids (VLCFA)	Lithium Heparin or EDTA Blood (4 mL)	For the investigation of peroxisomal disorders.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Vitamin A	SST, Plain or Lithium Heparin Blood (5 mL)	Protect sample from light. Cholesterol to be analysed at the same time to calculate Vitamin A/cholesterol ratio.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
Vitamin B1 (Thiamine)	Lithium Heparin Blood (5 mL)	The safest confirmatory test of Vitamin B1 deficiency is a trial of vitamin supplementation. Please contact laboratory before sending sample.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Vitamin B12	SST, Plain or Lithium Heparin Blood (5 mL)	More information.	Biochemistry Referred Test St Helier Hospital	2
Vitamin B6	Please contact the laboratory (7704) to discuss Blood	The safest confirmatory test of Vitamin B6 deficiency is a trial of vitamin supplementation. Please contact laboratory before sending sample. Please note that this test is not routinely available and the referral laboratory only accepts samples during normal working hours Monday to Friday.	Biochemistry Referred Test Barts and the Royal London NHS Trust	21 to 28
Vitamin D (25 OH)	SST or Plain Blood (5 mL)	Vitamin D and Bone Health: A Practical Clinical Guideline for Patient Management (2013) - National Osteoporosis Society	Biochemistry Referred Test St Helier Hospital	21 to 28
Vitamin E	SST, Plain or Lithium Heparin Blood (5 mL)	Protect sample from light. Cholesterol to be analysed at the same time to calculate Vitamin E/cholesterol ratio.	Biochemistry Referred Test Barts and the Royal London NHS Trust	14 to 21
VMA	Acidified 24hr Urine 24hr Urine	Refer to Metadrenalines. More details in the Endocrinology E-Book.	Biochemistry Referred Test St Helier Hospital	7
Von Willebrand's Factor	Citrate Blood (4 x 2.7 mL)	Must be received on day of collection. Requires four 2.7ml samples. Please contact the department if paediatric samples are to be taken. Test performed at Haemophilia Centre, Churchill Hospital	Coagulation (7695)	56
White cell enzymes (WCE)	Lithium Heparin Blood (5 mL)	Contact Biochemistry before sending sample. Samples can only be taken Monday - Thursday during normal working hours. A specific enzyme profile is performed according to clinical details.	Biochemistry Referred Test Barts and the Royal London NHS Trust	28 to 56
Zinc	Trace Metals Blood (5 mL)	For assessment of zinc status in TPN patients please consult the TPN team	Biochemistry Referred Test Barts and the Royal London NHS Trust	21

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